20°C | Protein Effects of Hormone Replacement Therapy |
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Protein Effects of Hormone Replacement Therapy An in-depth proteomic analysis of the sera of 50 participants from the Women's Health Initiative (WHI) hormone replacement therapy trial provides some explanations for the trial's clinical results. Samir Hanash, M.D., Ph.D., from the Fred Hutchinson Cancer Research Center, Seattle, worked with a team of researchers to identify and quantify proteins from 2,576,869 mass spectra, the largest serum protein data set obtained from a human observational study or clinical trial to date. He said, "Remarkably, as many as 10 percent of plasma proteins analyzed were found to be affected by estrogen hormone replacement therapy in post-menopausal women. These changes indicate a substantial effect on coagulation and metabolic proteins that may explain the increased risk of venous thromboembolism and stroke, and the reduced risk of fracture, found in the WHI trial." The authors used baseline and one-year post-treatment sera samples from 50 women in the WHI trial, separated into five experimental pools. The average age of the subjects was 61.4 years. There were no statistically significant differences in any baseline characteristics between pools. Using the samples gathered one year after the initiation of therapy, they identified changes in serum levels of proteins directly involved in processes as disparate as osteogenesis, blood vessel morphogenesis, blood pressure regulation, immunity, inflammation and coagulation. |