Introducing the 'MYC' Test (Microdeletion in the Y-Chromosome) for the first time in India. In Collaboration with Andrology Laboratory Services, Incorporated, Chicago, USA.

1. What is the 'MYC' Test?

2. Why test for 'MYC'?

Infertile men with this condition don't need further evaluations or therapy since no treatment will alleviate this congenital cause.

Also, to warn prospective ICSI patients that their condition is transmissible to their sons.

3. What is 'Microdeletion in Y-chromosome'?

Azoospermia and can have many causes, including congenital factors, of these inherited determinants, the deletions in the Y-chromosomes(Yq) is more frequent.The genes playing important roles in spermatogenesis are located on the long arm of the Y-chromosomes or Yq 11.23. Consequently, any missing genetic material from within this area can directly result in Azoospermia or severe Oligospermia.

Should such a chromosomal dysfunction occur, the aliment has been categorised as a "microdeletion in the Y-chromosome", or "MYC" ("MIKE") for short.However, deletions of Yq that are large enough to be visible on karyotyping are common in infertile males.

4. Who needs testing for 'MYC'?

Studies have demonstated that some infertile men with idiopathic Azoospermia or severe Oligozoospermia carry micro deletions in Yq not present in their father or brothers.

More importantly, the trasmission of such microdeletions from a severely Oligospermic man to his ICSI-derived son have been documented. In other words, father and son shared identical genetic defects. The grand father, however, was fertile and did not have microdeletions. These facts and other related findings show that ICSI transmits de novo microdeletions.

All patients with azoospermia or severe azoospermia should be tested for microdeletion in the Y-Chromosomes for two primary reasons :

	To determine the etiology of the azoospermia or severe oligozoospermia
	To warn the prospective ICSI patients with Azoospermia or severe oligozoospermia that their condition if proven to be caused by microdeletions, transmissible.

5. What is the 'Incidence of MYC'?

Estimates reports that one in five men (range 3 to 30%) with idiopathic Azoospermia or severe Oligozoospermia have such microdeletions.

6. How is the 'MYC test' performed?

A polymerase chain reaction(PCR) amplification, using primers for Y-specific sequence tagged sites, provides a rapid method for the screening of microdeletions in men with idiopathic inferility.

7. How many Chromosomal sites are analyzed?
       

Eighteen chromosomal sites are analyzed.The sites analyzed include the regions which flank AZFb, AZFc and AZFd, in addition to DAZ, KAL-Y, SMCY and various flanking loci for key spermatogenesis related genes.specifically, these eighteen sites are :

AZFa: DYS271, KAL-Y
AZFB: DYS212, SMCY, DYS215, DYS218, DYS219, DYS221
AZFd: DYS223, DYF51S1, DYS237, DYS236

8. What is required for "MYC" test?

A saliva sample, taken by the patient with the help of cotton swab, its all that's needed.

To sample a "MYC" test sampling kit,
Call : 2481255

or write to:
Rotunda - Hygeia
The Center For Human Reproduction & Women Care
Post Office Lane,
Qadri house,
Rajbagh Srinagar.
Kashmir ( J & K) 190 001

E-mail : contact@rotunda-hygeia.com